Overview

Greetings. Welcome to the Hesselberth lab in the Biochemistry and Molecular Genetics department at the University of Colorado School of Medicine. We develop molecular methods to study DNA, RNA and proteins, and apply them to study specific biological problems. We collaborate extensively with others to apply these methods as broadly as possible.

DNA modification and mutation. We are developing genome-scale methods for studying how DNA modifications lead to mutation. Our new Excision-seq and Ribose-seq methods enable high resolution mapping of DNA modification throughout the genome. We aim to use these methods to dissect the mutational process, to understand how hot spots of mutation form in the human genome. We are currently using Excision-seq to study uracilcation of HIV genomes during infection, and mammalian DNA demethylation. We are also extending the method to characterize other types of DNA damage in genomes, including sugar and phosphate modifications.

RNA end modification and fate. We have developed methods to identify RNAs with "dirty" ends that are generated by programmed or unintentional RNA cleavage. We discovered that eukaryotic RNA end modification governs the fate of RNA fragments, poising them for further processing or decay. Moreover, competition between RNA repair and decay regulates an important cellular homeostasis pathway, suggesting that this mode of regulation may be widespread. We are currently developing new methods for identifying RNA with specific end modifications, and are applying these new methods to identify new RNA end modificaiton substrates, and to understand how these modifications impact RNA fate.


News

Congratulations Dr. Sally Peach! [2016 Apr 11]
Welcome to rotating students Laura White and Mandy Richer. [2015 Aug 30]
Several manuscripts published with Sandy Martin, Dave Barton, Francesca Storici and Marc Hammarlund [2015 Feb 14]
Recruiting a new genomics faculty member [2014 Aug 31]
Sally passed her comprehensive exam! [2014 Aug 16]
Jay, Sally and Suzi attended the GSA Yeast meeting in Seattle. [2014 Jul 29]
Jay gave a talk at the FASEB RNA Decay meeting in Big Sky, MT [2014 Jul 6]
Patrick Cherry joins the lab! [2014 Jul 1]
Our manuscript describing Excision-seq is published in Genome Research. [2014 Jul 1]
Our manuscript describing a new mode of branched RNA turnover is published in RNA. [2014 Apr 1]
Our study with Dave Barton and Daphne Cooper to globally map RNase L substrates is published in NAR [2014 Apr 1]